Following the increase in population and the urbanization, there is a need for a public transportation system to facilitate the movement of citizens and access to their activities and services. One of the main types of public transport systems is the metro that has an effective role in reducing traffic flow of cities. So, suitable location of metro stations with regard to the surrounding environment can play an important role in increasing the efficiency and performance of the metro system. The purpose of this paper is comparative anslysis of Imam-Hossein and Jaber-Ansari metro stations in Isfahan and their environmental quality. In this regard, the required information was collected through documentary and field studies (observational, questionnaire and interview). Also, for this purpose, random sampling method was used with unlimited statistical population and 121 questionnaires were distributed. In order to analyze the components and measures, one-sample T-test and Yu-Mann-Whitney test in SPSS software were used. As well, we used AHP hierarchical method through Expert Choice software in order to determine the final status of the two stations. The results indicate that based on the components and related measures of security, justice, safety, economic dynamism, social dynamics, user and access, as well as the importance of each of them, in general, Imam-Hossein station is in better condition than the station Jaber-Ansari, and this situation is mainly due to the station of Imam Hussein is in one of the most important and vibrant centers of the city and the station of Jaber Ansari is at the core of a residential neighborhood.